Detección del SARS-CoV-2 en células renales del aparato yuxtaglomerular. Implicaciones para la patogenia de la COVID-19
Keywords:
SARS-CoV-2; COVID-19; yuxtaglomerular apparatus; renal interstitial fibrosisAbstract
Introduction: Extrapulmonary infection sites of SARS-CoV-2 might contribute to COVID-19 pathogenesis. Particularly, infection and injury of renal tissue has been associated with morbidity and mortality of COVID-19. So, autopsy analysis of deceased individuals with COVID-19 and the possible impact of SARS-CoV-2 in various organs are worth to understand viral spread and the pathophysiological mechanisms of infection.
Previously, we have shown SARS-CoV-2 in tissue samples of deceased cases with COVID-19 and its relationship with host factors. This work aimed and focused on SARS-CoV-2´s detection in additional samples of renal tissues from individuals who died with COVID-19.
Materials and methods: Renal tissue samples from autopsies of 4 deceased individuals with COVID-19 were analyzed. They were studied through optical and fluorescence confocal microscopy using antibodies specific for SARS-CoV-2.
Results: Histological analysis with Picro Mallory staining showed the presence of interstitial fibrosis in all samples. This finding was related with detection of SARS-CoV-2 in cells important for both renal functions and homeostasis maintenance of tissues and organs, such as interstitial or fibroblast-like cells and cells of yuxtaglomerular complex.
Conclusion: These results may have implications for deregulation of renin-angiotensin-aldosterone system (RAAS) by SARS-CoV-2 and fibrosis development
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